Plug-and-Play 3D localization microscopy
Cohen, O. R.; Xiao, D.; Kedem, R. O.; ALALOUF, O.; Prakash, J.; NAKATANI, Y.; Gustavsson, A.-K.; Shechtman, Y.
Show abstract
Point-spread-function engineering by depth-encoding phase masks enables volumetric super-resolution imaging by 3D single-molecule localization microscopy (SMLM) but usually requires cumbersome relay optics. We demonstrate simple 3D SMLM implementation by phase mask insertion directly into the infinity space of a commercial microscope, along with appropriate computational compensation for field-dependence. The entire inserted component, including the phase mask, is 3D printing-based.
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