Molecular organization of the eukaryotic secretory pathway
Mycroft-West, C. J.; Yee, N. B.- y.; Leanca, M. A.; Darrow, M. C.; Wu, L.
Show abstract
The eukaryotic secretory pathway is an essential subcellular network comprised of the endoplasmic reticulum (ER) and Golgi organelles. It mediates the trafficking of protein, lipid and other biomolecular cargoes throughout the cell, and is also the site of enzymatic modifications such as glycosylation that are vital for biological function. Complex interactions between resident functional proteins and cargo biomolecules drive myriad activities within the secretory pathway, but how these components organize within native milieu is still poorly understood. Here, we used cryo-electron tomography to survey the HeLa secretory pathway, revealing detailed features of its organellar structure. By quantitatively mapping protein distributions, we find that interactions within the HeLa secretory pathway are dominated by small dimeric complexes, stochastically distributed across both ER and Golgi membranes. Our survey of secretory pathway biomolecules provides new insights into putative trafficking and modification mechanisms, and highlights links between molecular organization and function within intracellular compartments.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Dynamic label-free analysis of SARS-CoV-2 infection reveals virus-induced subcellular remodeling. 95%
- Surfaceome dynamics during neuronal development and synaptic plasticity reveal system-wide surfaceome reorganization independent of global proteostasis 95%
- Cryo-electron tomography pipeline for plasma membranes. 95%
Similar papers in this journal
- Morpho-functional characterization of the endo-lysosomal system by high-throughput correlative light-electron microscopy 96%
- SHIP164 is a Chorein Motif Containing Lipid Transport Protein that Controls Membrane Dynamics and Traffic at the Endosome-Golgi Interface. 95%
- The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER-Golgi contact sites 94%
Similar papers in this journal
Similar papers in this journal
- Dynamic Palmitoylation Events Following T-Cell Receptor Signaling 94%
- The SNARE Sec22b regulates phagosome maturation by promoting ORP8-mediated PI(4)P exchange at ER-phagosome contact sites. 94%
- Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.