Mechanically induced pre-mitotic nuclear deformations promote nuclear rupture through lamin B1 depletion
Hensgens, M. N. F.; Sun, H.; Peters, B.; de Gruiter, H. M.; Slotman, J. A.; Idema, T.; Geertsema, H.
Show abstract
Disassembly of the nuclear lamina is essential for successful mitosis in eukaryotic cells, but the mechanism that triggers the lamina breakdown remains unclear. Using 3D fluorescence microscopy imaging combined with 3D curvature analysis, we found that microtubule-dependent invaginations trigger lamina remodelling and lamin B1 network disruption. Monte Carlo simulations provide mechanistic insight into how those invaginations induce a mechanical discontinuity between isotropic expansion and anisotropic shrinking forces at the rim of the nucleus, which correlated with lamin B1 depletion sites and the location of cytoplasmic protein influx within the nucleus. Our findings suggest that pre-mitotic modifications of the lamina function as a mechanical trigger to facilitate nuclear rupture and are thereby a key driver of mitotic entry, complementing established biochemical pathways.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Nuclear blebs are associated with destabilized chromatin packing domains 93%
- Rear cortex contraction aids in nuclear transit during confined migration by increasing pressure in the cell posterior 93%
- Paclitaxel compromises nuclear integrity in interphasethrough SUN2-mediated cytoskeletal coupling 93%
Similar papers in this journal
- Actin contraction controls nuclear blebbing and rupture independent of actin confinement 94%
- Physicochemical mechanotransduction alters nuclear shape and mechanics via heterochromatin formation 92%
- Lamin A/C Deficiency Enables Increased Myosin2 Bipolar Filament Ensembles Which Promote Divergent Actomyosin Network Anomalies Through Self Organization 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.