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Prenatal cannabis exposure affects human fetal neurodevelopment: anintegrated multi-omics study

Kahlert, G.; Chen, X.; Bammler, T. K.; MacDonald, J. W.; Benson, L. S.; Glass, I. A.; Dempsey, J. C.; Singh, D. K.; Prasad, B.; Unadkat, J. D.

2026-07-20 neuroscience
10.64898/2026.07.15.738779 bioRxiv
Show abstract

Prenatal cannabis use is on the rise, and observational studies suggest that such use results in neurodevelopmental deficits in the offspring. Because observational studies can be confounded by unaccounted factors, we studied the neurodevelopmental consequences, at the molecular level, of prenatal cannabis use. We applied an integrated multi-omics approach, combining transcriptomics and global proteomics, to first trimester (T1) and second trimester (T2) human fetal brains from pregnancies with and without documented maternal cannabis exposure and no use of drugs of abuse. Prenatal cannabis exposure produced minimal molecular effects in female T1 fetal brains but induced pronounced system-level disruption in male T2 fetal brains. These disrupted pathways have molecular signatures linked to neurodevelopmental and neuropsychiatric disorders, including autism spectrum disorder, schizophrenia-related pathology, and disorders of cortical connectivity, raising significant concerns of prenatal cannabis use.

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