High-resolution single-molecule replication profiling of the human genome
Tourancheau, A.; Rojat, V.; Ciardo, D.; Proux, F.; Lacroix, L.; Arbona, J.-M.; Audit, B.; Hyrien, O.; Le Tallec, B.
Show abstract
Despite the considerable progress made in recent years thanks to the rise of long-read sequencing, high-resolution, single molecule (SM)-based replication profiling of large genomes has remained out of reach. Here, we present a replication labelling strategy consisting in repeated pulse-labelling of asynchronously growing cells with the thymidine analogue bromodeoxyuridine (BrdU) that greatly enhances the number of detectable replication tracks. We show that the multipulse BrdU labelling protocol, in association with ForkML, a machine-learning method translating BrdU signals in nanopore reads into oriented and positioned replication forks, enables the establishment of a SM replication map of the entire human genome at kilobase resolution.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Fast Fourier Transform is a training-free, ultrafast, highly efficient, and fully interpretable approach for epigenomic data compression 94%
- Three-dimensional super-resolution fluorescence imaging of DNA 94%
- A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.