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Nuclear-BRITE: an enhanced MS2 system for live-cell imaging of nuclear RNAs
Cawte, A. D.; Cihlova, B.; Brockdorff, N.
2026-07-08
cell biology
10.64898/2026.07.07.736975
bioRxiv
Show abstract
The MS2 system has been widely adopted for live-cell single molecule imaging of mRNA, but is of limited applicability for RNAs present in the cell nucleus due to excess nuclear-localised fluorescent capsid protein. Here we describe Nuclear-BRITE, an improved methodology that reduces nuclear background signal, enabling fast live-cell imaging of a wide range of endogenously tagged nuclear RNAs at single-molecule resolution without perturbing their abundance, localisation or function.
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