The TIRR-53BP1 axis controls PLK1 spatiotemporal regulation during mitosis
Sarkar, A.; Roychoudhury, S.; Choe, K. N.; Umbreit, N. T.; de Boer, H. R.; He, Y. J.; Tomasik, B.; Vugt, M. A. T. M.; Pellman, D.; Chowdhury, D.; Spektor, A.
Show abstract
p53-binding protein 1 (53BP1) is a key mediator of the DNA damage response and genome stability. While its interphase function is well-characterized, its mitotic role remains less understood. Here we show that aberrant activation of 53BP1 through the loss of its negative regulator, TIRR, leads to mitotic abnormalities including altered spindle geometry, kinetochore-microtubule (k-MT) attachment errors and whole chromosome missegregation. We demonstrate that loss of TIRR results in excess interaction between 53BP1 and the key mitotic kinase Polo-like kinase 1 (PLK1), altering PLK1s activation, spatial distribution, and its interaction with known PLK1 substrates at multiple mitotic stages. Moreover, due to PLK1s established role in CENP-A loading, hyperactivation of 53BP1 compromises CENP-A loading, triggers gradual loss of CENP-A from centromeres and generates severe kinetochore assembly defects. These findings uncover a non-canonical mitotic function of 53BP1 as a key regulator of PLK1 activity and chromosome segregation fidelity.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.