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AutoBlot: deterministic single-cell western blotting reveals proteomic diversity in rare cell populations

Deroy, C.; Islam, M. N.; Goldhammer, N.; Jiang, L.; Rosenbluth, J. M.; Herr, A. E.

2026-06-23 bioengineering
10.64898/2026.06.22.733890 bioRxiv
Show abstract

Single-cell western blotting (scWB) provides quantitative, molecular-mass-resolved protein measurements but relies on Poisson-limited gravity settling, requiring [~]106 starting cells with <37% microwell occupancy. AutoBlot replaces stochastic loading with piezoelectric dispensing, achieving [~]98% occupancy and deterministic cell-bead co-isolation from as few as 10,000 starting cells. Profiling patient-derived breast organoids (PDOs), we detect BRCA1-associated proteomic shifts, menopausal-status-dependent ER expression differences, and systematic differences between surface and intracellular lineage classification of mammary epithelial cells in organoid culture.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.