Polychrome activity profiling distinguishes Cys proteases and their isoforms in plants
Zheng, K.;Schuster, M.;Sanguankiattichai, N.;Kessenbrock, T.;Kaiser, M.;Hoorn, R.
Show abstract
Activity-based profiling with fluorescent probes is a powerful tool for the functional characterization of whole enzyme classes in crude proteomes. Here, we discovered that probe cocktails consisting of the E-64 warhead carrying different fluorophores via short linkers distinguishes the labeling of papain-like cysteine proteases and their isoforms because of their differential affinity to these probes. This causes polychrome labeling with specific apparent colors for different protease families and isoforms. Polychrome labeling revealed differential labeling of isoforms of RD21-like proteases carrying C-terminal granulin domains. Molecular modeling of the RD21 isoforms revealed that the C-terminal granulin may create a fluorophore-binding pocket with the substrate-binding groove, implying that the granulin domain influences substrate selectivity. The concept of polychrome labeling may be widely applicable to other chemical probes for the characterization of protein families in all kingdoms of life.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems 97%
- Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors 95%
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization 95%
Similar papers in this journal
- Unbiased proteomic and forward genetic screens reveal that mechanosensitive ion channel MSL10 functions at ER-plasma membrane contact sites in Arabidopsis thaliana 93%
- An increase of NPY1 expression leads to inhibitory phosphorylation of PIN-FORMED (PIN) proteins and suppression of pinoid (pid) null mutants 93%
- Guanidine Production by Plant Homoarginine-6-hydroxylases 93%
Similar papers in this journal
- Catch & Release - rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach 96%
- Localization of four class I glutaredoxins in the cytosol and the secretory pathway and characterization of their biochemical diversification 95%
- The necrotrophic effector ToxA from Parastagonospora nodorum interacts with wheat NHL proteins to facilitate Tsn1-mediated necrosis 94%
Similar papers in this journal
- Disrupted Nitric Oxide Homeostasis Impacts Fertility Through Multiple Processes Including Protein Quality Control 95%
- Phosphate starvation regulates cellulose synthesis to modify root growth 94%
- The secreted hypersensitive response inducing protein 1 from Botrytis cinerea displays non-canonical PAMP-activity 94%
Similar papers in this journal
- Rational domestication of a plant-based recombinant expression system expands its biosynthetic range. 94%
- Phenylacetic acid metabolism in land plants: novel pathways and metabolites 94%
- Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.