Galectin-1 identifies a unique subpopulation of highly invasive glioblastoma cells and enables their migration
Sanazzaro, T.;Cotner, M.;Arvinth, N.;Brock, A.;Seidlits, S.
Show abstract
Glioblastoma (GBM), the most common primary brain tumor, is characterized by extensive infiltration into surrounding brain tissue. GBM tumors exhibit substantial intratumoral heterogeneity making it difficult to identify and target invasive cell subpopulations. Here, we use an in vitro model of the mechanical transitions at the tumor-brain interface to isolate highly invasive GBM cells from populations derived from unique patient tumors for downstream transcriptomic analysis or further culture. Using single-cell RNA sequencing combined with cell barcodes we were able to trace distinct cell lineages during migration and identify an intrinsically invasive subpopulation. This invasive subpopulation exhibits a distinct pre-invasive transcriptomic profile characterized by overexpression of galectin-1, a {beta}-galactoside binding protein. Our findings reveal galectin-1 overexpression is an innate characteristic of invasive GBM subpopulations, where expression level positively correlates with invasion rate and inhibition of galectin-1 binding to cell surface glycoproteins effectively prevented migration. While some studies have reported that galetcin-1 aids in cell migration, this study identifies and confirms that galectin-1 expression is a pre-existing characteristic of invading GBM cells and a target to prevent tumor recurrence.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Simulating the human tumor microenvironment in colorectal cancer organoids in vitro and in vivo 93%
- A 3D adrenocortical carcinoma tumor platform for preclinical modeling of drug response and matrix metalloproteinase activity 93%
- Functional maturation of human neural stem cells in a 3D bioengineered brain model enriched with fetal brain matrix 93%
Similar papers in this journal
Similar papers in this journal
- Versatile roles of Annexin A4 in ccRCC: impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment 92%
- Mesenchymal-epithelial transition reduces proliferation but increases immune evasion in tumor spheroids 92%
- Multicellular Organoids of the Neurovascular Blood-Brain Barrier: A New Platform for Precision Neuronanomedicine 92%
Similar papers in this journal
Similar papers in this journal
- Mechanical environment afforded by engineered polymer hydrogel critically regulates survival of neural stem cells transplanted in the injured spinal cord via Piezo1-mediated mechanotransduction 93%
- Extracellular matrix viscoelasticity regulates mammary branching morphogenesis 92%
- Microphysiological uremia model reveals biophysical potentiators of vascular dysfunction 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.