Quantitative insights into the role of phages and plasmids in the persistence of nontuberculous mycobacteria in chloraminated drinking water
Lee, S.; Langenfeld, K.; Potgieter, S.; Ferdous, S. M.; Vasagiri, S.; Bastien, G. E.; Duhaime, M.; Wigginton, K.; Raskin, L.; Hegarty, B.
Show abstract
Nontuberculous mycobacteria (NTM) are opportunistic pathogens that persist in chloraminated drinking water systems, yet the roles of phages and plasmids in their persistence remain largely unexplored. Using genome-resolved and quantitative metagenomics, we characterized NTM, phages, prophages, and plasmids in a chloraminated building plumbing system. Bacterial metagenome-assembled genomes (MAGs) and viral operational taxonomic units (vOTUs) were quantified at mean concentrations of 8.41 * 10^7 and 8.00 * 10^8 copies/L, respectively, including seven NTM MAGs at a mean total concentration of 4.01 * 10^5 copies/L. NTM concentrations were highest at the site with the lowest bacterial and viral diversity. Predicted NTM-infecting virus concentrations were inversely related to NTM concentrations across sites, suggesting complex phage-host dynamics that warrant direct experimental investigation. NTM, putative phages, prophages, and plasmids encoded functions related to disinfectant tolerance, stress response, metal resistance, and secretion. These findings identify phage interactions, prophages, and plasmids as overlooked genomic and ecological dimensions of NTM persistence in engineered water systems.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Horizontal gene transfer and CRISPR targeting drive phage-bacterial host interactions and co-evolution in pink berry marine microbial aggregates 95%
- With a pinch of salt: metagenomic insights into Namib Desert salt pan microbial mats and halites reveal functionally adapted and competitive communities 94%
- Cryptic cycling by electroactive bacterioplankton in Trout Bog Lake 94%
Similar papers in this journal
- Longitudinal metatranscriptomic sequencing of Southern California wastewater representing 16 million people from August 2020-21 reveals widespread transcription of antibiotic resistance genes. 97%
- Low diversity and microdiversity of comammox bacteria in wastewater systems suggests wastewater-specific adaptation within the Ca. Nitrospira nitrosa cluster. 94%
- Building plumbing influences the microdiversity and community assembly of the drinking water microbiome. 94%
Similar papers in this journal
- Life on the edge: microbial diversity, resistome, and virulome in soils from the Union Glacier cold desert 95%
- New insights into functional divergence and adaptive evolution of uncultured bacteria in anammox community by complete genome-centric analysis 94%
- Aquatic microbial community is partially functionally redundant: insights from an in situ reciprocal transplant experiment 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.