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From the scanner to daily life: Neural expression of the Picture-Induced Negative Emotion Signature is associated with real-world daily and momentary negative affect in midlife adults

Wu-Chung, E. L.; Eckerle, W. D.; Marsland, A. L.; Kraynak, T. E.; Kamarck, T. W.; Gianaros, P. J.

2026-06-08 neuroscience
10.64898/2026.06.03.729931 bioRxiv
Show abstract

Functional magnetic resonance imaging (fMRI) is widely used to map brain systems that process affective stimuli and predict concurrent affective experiences in the scanner. However, the extent to which affect-related fMRI activity in the scanner predicts affective experiences in daily life remains unclear. This study examined whether a multivariate brain pattern previously validated to predict negative affect to emotional images during fMRI testing (the Picture-Induced Negative Emotion Signature; PINES) associates with daily negative affect measured over 7-10 days via daily diaries and ecological momentary assessment. Among 287 midlife adults, those exhibiting greater PINES pattern expression showed greater daily (end-of-day) and hourly negative affect. These findings were not explained by demographic factors and trait negative affect. A cross-validated whole-brain pattern of negative affect generalizes beyond the fMRI testing context to predict negative affect severity in daily life.

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