Satellite DNA Editing Enables Meiosis-Independent Chromosome Engineering
Zhou, R.; Chen, M. S. S.; Drowns, M. R.; Mailloux, K.; Yin, K.; Vaillancourt, B.; Buell, C. R.; Tsai, C.-J.
Show abstract
Chromosome{square}scale genome engineering in plants typically relies on locus-specific recombination during meiosis to select desirable outcomes, limiting its application in species with long generation times. Here, we show that CRISPR targeting of abundant satellite DNA enables multiplexed chromosome restructuring in aspen, generating diverse large-scale structural variants in the first generation. These megabase rearrangements remain mitotically stable through clonal propagation and regeneration with no obvious growth defects.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A highly efficient CRISPR-Cas9-based gene editing system in oat (Avena sativa) 95%
- Genome-wide analyses of PAM-relaxed Cas9 genome editors reveal substantial off-target effects by ABE8e in rice 94%
- A near gap-free haplotype-resolved genome assembly of Zoysia japonica uncovers intra-subgenomic gene expression and regulatory variation 93%
Similar papers in this journal
Similar papers in this journal
- An Evolutionarily Conserved Receptor-like Kinases Signaling Module Controls Cell Wall Integrity During Tip-Growth 92%
- The genomes and epigenomes of aquatic plants (Lemnaceae) promote triploid hybridization and clonal reproduction 91%
- Apical and basal auxin sources pattern shoot branching in a moss 91%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.