Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution
Lamb, J. R.; Cardoso Mestre, M.; Fenwyn Longrin, K.; Bhat, P.; Stevenson, M.; Rhodes, A. D. Y.; Gosieniecka, J.; Redmond, L. C.; Higgins, C. A.; Rodriguez-Rodrigues, N.; Lancaster, M. A.; Manton, J. D.
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We present cleared-tissue direct-view oblique plane microscopy (CtDvOPM), which enables optically sectioned subcellular resolution imaging of centimetre-scale tissues at high-throughput over the full range of clearing media refractive indices (n = 1.33-1.56). CtDvOPM can image conventionally-mounted expanded, aqueous or non-aqueous cleared tissue samples at up to 2 {micro}m lateral by 14 {micro}m axial resolution over a 10 mm x 10 mm x 25 mm sample volume without image tiling, at up to 400 million voxels per second.
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