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Mechanism for the initiation of co-transcriptional pre-60S assembly

Piwowarczyk, R.; Klinge, S.

2026-05-22 biochemistry
10.64898/2026.05.22.727207 bioRxiv
Show abstract

Eukaryotic ribosomal large subunit (60S) assembly requires an internal transcribed spacer 2 (ITS2) to license both nucleolar and nuclear pre-60S assembly intermediates. The underlying molecular mechanisms responsible for nucleation of pre-60S assembly, quality control, and installation of ITS2 during co-transcriptional stages remain unknown. Here we report the earliest co-transcriptional assembly intermediates of the eukaryotic 60S subunits. Together with biochemical assays, our data reveal the architecture of co-transcriptional pre-60S assembly initiation and progression, as well as the molecular logic of an assembly checkpoint. This study highlights an evolutionary solution by which complex RNA folding processes can be parallelized and integrated via biological AND-gating.

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