Dual histone methylation reader ZCWPW2 links histone methylation to initiation of meiotic recombination
Yuan, S.; Wang, S.; Bao, Z.; Wang, Z.; Shangguan, K.; Yan, Y.; Shi, Y.; Feng, X.; Huang, C.; Fang, M.; Zhao, H.; Zhao, S.; Liu, H.; Chen, Z.-J.; Huang, T.
Show abstract
Meiotic homologous recombination initiates with the formation of programmed DNA double-strand breaks (DSBs) by complexes comprising SPO11 and accessory proteins at discrete sites called recombination hotspots. In mammals, PRDM9-dependent H3K4me3 and H3K36me3 define recombination hotspots, but how these epigenetic characteristics determine the physiological DSB formation remains unknown. Here we show that dual histone methylation reader ZCWPW2 can recognize H3K4me3 and H3K36me3 marks in testis. The binding activity of ZCWPW2 to histone methylation is dependent on PRDM9 function. Moreover, we find the epigenetic writer-reader axis PRDM9-ZCWPW2 is essential for DSB formation at meiotic recombination hotspots, which may be partly explained by the finding that ZCWPW2 can physically interact with HORMAD1, IHO1, MEI4, and REC114. Finally, the absence of ZCWPW2 leads to disrupted chromosomal synapsis and recombination, thereby obstructing meiotic progression. Taken together, our findings provide new insights into how histone modifications and their associated regulatory proteins collectively regulate meiotic homologous recombination initiation.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The combined action of CTCF and its testis-specific paralog BORIS is essential for spermatogenesis 96%
- RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis 96%
- Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation 96%
Similar papers in this journal
Similar papers in this journal
- A meiosis-specific factor MRM/C19orf57 modulates localization of RAD51 and DMC1 recombinases to DSBs in mouse meiotic recombination 96%
- YTHDC2 Is Essential for Pachytene Progression and Prevents Aberrant Microtubule-Driven Telomere Clustering in Male Meiosis 96%
- C2cd6-encoded CatSper{tau} Targets Sperm Calcium Channel to Ca2+ Signaling Domains in the Flagellar Membrane 94%
Similar papers in this journal
- PNLDC1 catalysis and postnatal germline function are required for piRNA trimming, LINE1 silencing, and spermatogenesis in mice 96%
- RDC complex executes a dynamic piRNA program during Drosophila spermatogenesis to safeguard male fertility 96%
- Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosis 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.