How many genes can CRISPR edit to engineer complex adaptations?

Polygenic traits require the coordinated effects of multiple genes. Such complex traits have been a long-term target of study for geneticists, but multiplex CRISPR--the editing of multiple loci in the genome via multiple guide RNAs--is in its infancy. Reviewing 106 plant studies using multiplex CRISPR, we find that the multiplexing capacity has doubled every 5.4 years; furthermore, a systematic experiment with 8, 16, and 24 simultaneous targets in Arabidopsis thaliana reveals that efficiency of 24-plex editing can reach up to 73% across over one hundred third-generation transformed plants sequenced. Our experiment revealed that the level of multiplexing, or the number of the targets, causes minor efficiency reduction compared to the other uncontrolled factors such as gRNA design or variation across plants. When we model the decay in editing efficiency as a function of the gRNA number, actual efficiency is higher than the expectation from both Cas9 competition interference and simple joint editing stochasticity models. Rather, efficiency decayed with diminishing interference with more gRNAs with substantial overdispersion attributed to other efficiency factors, such as PAM identity. We predict that editing close to 100 genes in a plant can be feasible with reasonably large plant screens; however, feasible and reliable polygenic genome engineering will necessitate developments outside of [insert novelty of this study in how multiplex CRISPR was implemented, here]. Author ContributionsM.E.-A. conceived the project and secured funding. M.E.-A. and M.Es. designed the experimental strategy. M.Es. established the multiplex CRISPR and transformation pipelines in the laboratory, propagation through the T1 and T2 generations, and oversaw the first amplicon sequencing. Y.P. established the in-house iSeq amplicon sequencing protocol and contributed to cloning and genotyping pilots. M.Es supervised K.P. to construct cloning, bacterial transformations, plant growth, floral-dip transformations, and selection of T1 plants. E.K. contributed to early amplicon genotyping. J.K. propagated and sampled the T3 and J.K. and J.M. conducted the final amplicon sequencing panel. J.K. and M.E.-A. performed gene editing variant mapping, dataset quality control, and summarized results from published multiplex CRISPR studies. M.E.-A. modeled editing efficiency. J.K and M.E.-A. generated figures and wrote the first draft. All authors revised and improved the manuscript. J.K. and M.Es. contributed equally to this work and are designated as co-first authors.