Two-dye-imager DNA-PAINT enables volumetric nanoscopy of expanded cells
Sauer, M.; Weingart, J.; Eilts, J.; Kiesel, C.; Perozhy, H.; Kollmannsberger, P.; Helmerich, D. A.; Doose, S.
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Refined single-molecule localization microscopy methods demonstrated superior localization precisions on isolated sample but remain limited by labeling density and imaging speed in cells. Here we combine expansion microscopy (ExM) with two-dye-imager (TDI)-DNA-PAINT to resolve fine molecular details of protein assemblies in [~]8-fold expanded cells with nanometer resolution. Using lattice light-sheet (LLS) microscopy, Ex-TDI-DNA-PAINT provides a robust platform for three-dimensional (3D) volumetric nanoscopy of the molecular organization of cells.
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