Methodological Evaluation and Data Resource for Andes Virus Sequencing Preparedness
Doherty, R.; Lewandowski, K.; Fenwick, A.; Everall, I.; Morley, D.; Hartman, H.; Staplehurst, S.; Kent, C.; Loman, N. J.; Quick, J.; Pullan, S. T.
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As part of preparedness activities supporting pathogens classified under the UK High Consequence Infectious Diseases (HCID) framework, we previously evaluated both a whole-genome tiling amplicon sequencing scheme and a pan-viral hybridisation capture approach (TWIST-CVRP) for sequencing Andes virus (ANDV). In light of the recent outbreak, we make available viral sequencing datasets generated using a historical ANDV isolate (Chile, 1997). In addition, we provide an evaluation of tiling amplicon scheme performance and present recommended primer updates informed by in silico comparison with the recently released outbreak genome. These datasets are intended to support benchmarking, validation, and optimisation of bioinformatic pipelines across the community.
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