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T cell-specific loss of IRF1 results in defective CD8 T cell activation and antitumor immunity

Shao, L.; Bannerjee, H.; Unal, E.; Mehta, I.; Das, J.; Banday, A. R.; Kane, L. P.; Sarkar, S. N.

2026-05-18 immunology
10.64898/2026.05.14.725206 bioRxiv
Show abstract

Interferon regulatory factor 1 (IRF1) has long been recognized as a tumor suppressor; however, recent studies have revealed context-specific and sometimes opposing roles in cancer progression. Here, we describe a T cell-specific mechanism underlying the antitumor activity of IRF1. Unlike germline Irf1-deficient mice, T cell-specific loss of IRF1 does not lead to a deficiency in cytotoxic CD8 T cells. Nevertheless, tumor burden remains elevated in these mice, associated with reduced CD8 T cell infiltration driven by impaired activation and proliferation in the absence of IRF1. Transcriptomic analysis of activated Irf1-deficient T cells identified NFATc1 as a key gene significantly downregulated upon IRF1 loss. Analysis of human melanoma datasets further corroborated this finding, highlighting a previously unappreciated role for IRF1 in regulating T cell activation and antitumor immunity.

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