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SUITPy: A Python-based toolbox for the analysis of cerebellar functional and anatomical imaging data across the human lifespan

Wang, Y.; Li, Y.; Arafat, B.; Ashkanichenarlogh, V.; Nettekoven, C. R.; Pinho, A. L.; Hernandez-Castillo, C.; Marquand, A. F.; Diedrichsen, J.

2026-05-18 neuroscience
10.64898/2026.05.14.724397 bioRxiv
Show abstract

The human cerebellum plays a central role in motor, emotional, and cognitive functions, and is implicated in many brain disorders. To improve the analysis of functional and anatomical imaging from the cerebellum, we introduce SUITPy, an improved and fully revised Python implementation of the widely used SUIT toolbox. For this new version, we developed a U-Net based model to automatically isolate the cerebellum from adjacent cortical tissue, which achieves higher fidelity than existing algorithms. The isolation works robustly without manual corrections for imaging data across the lifespan. We show that isolation and subsequent normalization to a cerebellum-only template lead to a more precise alignment of cerebellar structures across participants compared to normalization using a whole-brain template. We also show the utility of the cerebellar mask to prevent contamination of cerebellar functional data from surrounding cortical structures. The toolbox also provides functionality for visualizing cerebellar data on a flatmap, along with a range of anatomical and functional cerebellar atlases, thereby offering an essential tool that enables accurate cerebellar analysis across the lifespan.

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