Turnip mosaic virus co-opts host RNA methylation to orchestrate plant infection
Hirt, H.; Almeida-Trapp, M.; Nawaz, K.; Secco, N.; Sheikh, A.
Show abstract
N6-methyladenosine (m6A) is a key RNA modification that regulates transcript stability and translation. However, its function in plant viruses remains largely unclear. Here, we show that the positive-sense single-stranded +ssRNA Turnip mosaic virus (TuMV) relies on the host m6A machinery to support efficient infection. Our findings uncover a previously unrecognized nuclear phase in the TuMV life cycle, during which viral RNA undergoes extensive methylation by host enzymes. We identify a complex and non-canonical methylation landscape on the TuMV genome, where canonical DRACH motifs are embedded within clusters of additional virus-specific non-canonical m6A sites. Notably, we also detect the presence of another RNA modification, m5C (5-methylcytosine), in close proximity to m6A-marked regions. This coordinated methylation landscape appears to be critical for efficient viral polyprotein synthesis. In its absence, the virus displays aberrant methylation and reduced infectivity as observed in m6A writer and reader mutants. Based on this, we propose a "seeding" model in which initial m6A deposition at canonical sites nucleates the formation of a broader network of m6A and m5C marks, likely guided by RNA structure rather than sequence motifs alone.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- N6-methyladenosine modification is not a general trait of viral RNA genomes 95%
- CLSY docking to Pol IV requires a conserved domain critical for small RNA biogenesis and transposon silencing. 95%
- Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis 95%
Similar papers in this journal
- A novel ilarvirus protein is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing 95%
- Characterisation of the Semliki Forest Virus-host cell interactome reveals the viral capsid protein as an inhibitor of nonsense-mediated mRNA decay 94%
- Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly. 94%
Similar papers in this journal
- Investigating molecular mechanisms of 2A-stimulated ribosomal pausing and frameshifting in Theilovirus 96%
- Timed chromatin invasion during mitosis governs prototype foamy virus integration site selection and infectivity 95%
- Visualizing the transcription and replication of influenza A viral RNAs in cells by multiple direct RNA padlock probing and in-situ sequencing (mudRapp-seq) 94%
Similar papers in this journal
Similar papers in this journal
- Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression 95%
- Principles of RNA recruitment to viral ribonucleoprotein condensates in a segmented dsRNA virus 95%
- Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underlies Arabidopsis response to viral infection 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.