Subnuclear cofactor partitioning underlies auxin-dependent transcriptional regulation
Hernandez-Garcia, J.; Dipp Alvarez, M.; de Roij, M.; Guillem-Bernal, M.; Roosjen, M.; Baltaci, Z.; Smith, H. W.; Pereira, D.; Borst, J. W.; Holehouse, A. S.; Lee, H. O.; Weijers, D.
Show abstract
Cellular and organismal function relies on the precise activation and repression of gene expression by DNA-binding transcription factors (TFs). Many TFs occur in large gene families, and a key question in biology is how divergent functions emerge in TF families during evolution. Here we discover the biochemical mechanism for transcriptional activation by the Marchantia polymorpha AUXIN RESPONSE FACTOR1 (MpARF1) TF, which relies on direct recruitment of the Mediator complex into subnuclear MpARF1 clusters. We find that the Mediator recruitment region was the evolutionary innovation that converted ARF repressors into activators, switching binding specificity from co-repressor to co-activator. We demonstrate that this evolutionary innovation can be recreated, thereby revealing a deeply conserved mechanism based on competition between ARF clusters at the heart of the transcriptional auxin response.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- The ALOG domain defines a new family of plant-specific transcription factors acting during Arabidopsis flower development 97%
- Cytosolic iron-sulfur protein assembly system identifies clients by a C-terminal tripeptide 97%
- Molecular convergence by differential domain acquisition is a hallmark of chromosomal passenger complex evolution 97%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.