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ILC3s are Required for Enterocyte Homeostasis to Food Intake

Lecuyer, E.; Guendel, F.; Cording, S.; Nigro, G.; Medvedovic, J.; Dulauroy, S.; Rincel, M.; Chassaing, B.; Langa-Vives, F.; Dejardin, F.; Moguel, H.; Eberl, G.

2026-04-22 immunology
10.64898/2026.04.20.719606 bioRxiv
Show abstract

Food provides nutrients that are selectively absorbed by the intestine, but, at the same time, may contain elements that challenge the intestinal barrier and induce post-prandial inflammation (PPI). How PPI is controlled in order to avoid pathological perturbation of homeostasis remains unclear. Here, we report that during fasting, enterocytes increase their absorptive potential and oxidative metabolism, a program that is largely reversed upon food intake of lipids that perturb the intestinal barrier and induce PPI. Such perturbation is countered by ILC3s, in the absence of which PPI increases, program reversal does not occur, and enterocytes engage into excessive oxidative metabolism. This enterocyte state leads to critical hypoglycemia as a consequence of decreased glucose absorption and increased insulinemia, recapitulating the pathological situation found in patients suffering from intestinal damage and sepsis. We hereby uncover a critical function for ILC3s in maintaining enterocyte homeostasis upon challenging food intake.

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