Back

Integrated mapping of human meniscus and cartilage eQTLs reveals shared and distinct osteoarthritis genetic drivers

Uchida, Y.; Fujii, Y.; Swahn, H.; Ueda, M. T.; Chiba, T.; Matsushima, T.; Naito, Y.; Nakamichi, R.; Takahashi, K.; Olmer, M.; The RE-JOIN Consortium Investigators, ; Lotz, M.; Kochi, Y.; Asahara, H.

2026-04-16 orthopedics
10.64898/2026.04.12.26350702 medRxiv
Show abstract

Osteoarthritis (OA) is a prevalent musculoskeletal disorder and a leading cause of global disability. Although meniscal damage is a major risk factor of OA pathogenesis, genetic regulatory studies have remained largely confined to articular cartilage. Here, we establish the first comprehensive expression quantitative trait locus (eQTL) map integrating whole-genome sequencing and bulk transcriptomics from human meniscus (n=112) and cartilage (n=113). Supported by single-nucleus multiomics (cartilage: 56,549 nuclei; meniscus: 34,343 nuclei), we uncovered highly tissue-specific genetic risk architectures. Colocalization with OA GWAS identified 27 meniscus-specific, 28 shared, and 20 cartilage-specific causal genes. Chromatin-informed fine-mapping and deconvolution elucidated distinct pathogenic mechanisms; notably, meniscus-specific signals converged on VEGFA via rare promoter variants and an enhancer in fibrochondrocyte progenitors, alongside a shared eQTL for CLEC18A. Exploratory analysis suggested candidate compounds to reverse pathogenic gene expression. Our findings underscore the meniscus as a distinct genetic driver, molecularly reinforcing OA as an entire joint organ failure.

Matching journals

The top 3 journals account for 50% of the predicted probability mass.

1
Nature
575 papers in training set
Top 0.4%
32.4%
2
Science
429 papers in training set
Top 3%
9.0%
3
Nature Communications
4913 papers in training set
Top 21%
9.0%
50% of probability mass above
4
Cell
370 papers in training set
Top 3%
6.2%
5
Nature Genetics
240 papers in training set
Top 1%
6.2%
6
Molecular Cell
308 papers in training set
Top 5%
2.6%
7
Science Advances
1098 papers in training set
Top 16%
1.8%
8
Advanced Science
249 papers in training set
Top 11%
1.7%
9
Science Translational Medicine
111 papers in training set
Top 3%
1.7%
10
Nature Cell Biology
99 papers in training set
Top 3%
1.6%
11
Nature Structural & Molecular Biology
218 papers in training set
Top 3%
1.5%
12
eLife
5422 papers in training set
Top 48%
1.3%
13
Cell Research
49 papers in training set
Top 2%
1.3%
14
Nature Neuroscience
216 papers in training set
Top 5%
1.3%
15
Developmental Cell
168 papers in training set
Top 9%
1.3%
16
Nature Medicine
117 papers in training set
Top 3%
1.2%
17
Proceedings of the National Academy of Sciences
2130 papers in training set
Top 39%
1.1%
18
PLOS Genetics
756 papers in training set
Top 12%
0.9%
19
Nature Biotechnology
147 papers in training set
Top 7%
0.9%
20
Neuron
282 papers in training set
Top 8%
0.9%
21
Journal of Clinical Investigation
164 papers in training set
Top 6%
0.8%
22
Nature Metabolism
56 papers in training set
Top 2%
0.8%
23
Cell Stem Cell
57 papers in training set
Top 2%
0.8%
24
Immunity
58 papers in training set
Top 4%
0.8%
25
Cell Genomics
162 papers in training set
Top 7%
0.7%
26
Cell Reports
1338 papers in training set
Top 36%
0.6%