Quantifying Brittle Crack Opening in Human Trabecular Bone Using Synchrotron XCT-DVC

IntroductionTrabecular bone exhibits brittle behaviour governed by microscale deformation and damage processes, yet quantitative characterisation of crack progression remains challenging because classical fracture mechanics approaches do not apply to architecturally discontinuous porous tissues. This study evaluates whether synchrotron X-ray computed tomography (XCT) combined with digital volume correlation (DVC) can provide a practical experimental approach for quantifying crack opening behaviour in human trabecular bone. MethodSemicylindrical specimens harvested from femoral heads of hip-fracture donors (n = 5) and non-fracture controls (n = 5) underwent stepwise three-point-bending during XCT imaging. Full-field displacement maps enabled direct measurement of crack mouth opening displacement (CMOD), crack length (a), and their ratio, CMOD/a, used here as a geometry-normalised comparative descriptor of brittle response. Automated crack segmentation using phase-congruency crack detection (PCCD) was compared against manual measurements. ResultsXCT-DVC successfully resolved three-dimensional displacement discontinuities during crack initiation and propagation in all specimens. Hip-fracture donors exhibited significantly lower critical crack-opening ratios (CMOD/a)* than Controls (0.31 vs 0.47; p = 0.008) and reached mechanical instability at lower applied loads, consistent with a more brittle structural response under this test configuration. Despite these differences, total crack extension ({Delta}a*) was similar between groups. Automated crack tracking using phase-congruency-based segmentation showed excellent agreement with manual measurements (r{superscript 2} = 0.98), confirming reliable extraction of crack geometry from DVC displacement fields. DiscussionThese results indicate that XCT-DVC can provide a practical approach for quantifying crack-opening behaviour in trabecular bone when classical fracture-mechanics parameters are not applicable in anatomically constrained specimens. The reduced critical crack-opening ratios and earlier instability observed in Hip-fracture donors are consistent with a more brittle comparative mechanical response that is not captured by crack extension alone. The strong agreement between automated and manual crack measurements further supports displacement-based descriptors as reliable comparative indicators of brittle behaviour in porous, architecturally discontinuous tissues. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=76 SRC="FIGDIR/small/714043v1_ufig1.gif" ALT="Figure 1"> View larger version (28K): org.highwire.dtl.DTLVardef@31c5d7org.highwire.dtl.DTLVardef@1b3d9a4org.highwire.dtl.DTLVardef@95df7borg.highwire.dtl.DTLVardef@1834216_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOGraphical abstractC_FLOATNO C_FIG