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Paternal behavior is controlled by preoptic Trpc5 neurons

Li, Y.; Liu, Q.; Wang, F.; McDermott, K. M.; Wang, M.; Deng, Y.; Yang, Y.; Liu, Y.; Cheng, J.; Sun, M.; Liu, X.; Jian, J.; Qiu, J.; Wu, X.; Xue, L.; Zhou, T.; Yang, Y.; Liu, H.; Tu, L.; Arenkiel, B. R.; Farooqi, S. I.; Xu, Y.

2026-03-23 neuroscience
10.64898/2026.03.20.713203 bioRxiv
Show abstract

Male parenting behavior is highly conserved and activated only after offspring are born. Here we show that the behavioral switch from infanticide in virgin male mice to caregiving in wild-type sires is associated with enhanced expression and activation of Transient receptor potential channel 5 (Trpc5) in estrogen receptor (Esr1)-expressing neurons in the hypothalamic medial preoptic area (MPOA). While selective deletion of Trpc5 from Esr1MPOA neurons diminishes paternal behavior in sires, overexpression of Trpc5 in these neurons converts the otherwise infanticidal virgin males to exhibit care to pups. Mechanistically, Trpc5-dependent changes in neuronal excitability underlie fatherhood-associated Esr1MPOA neuron plasticity. Notably, Trpc5 overexpression also enhances escape behavior and elicits exploratory diving behavior, suggesting that these neurons control a broader adaptive parenting response in males. These findings establish a Trpc5-dependent MPOA neural signal as a critical regulator of paternal behavior.

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