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Engram and neural underpinning dynamics of the long-lasting childhood olfactory memory

Dejou, J.; Athanassi, A.; Brunel, T.; Thevenet, M.; Didier, A.; Mandairon, N.

2026-03-23 neuroscience
10.64898/2026.03.19.712996 bioRxiv
Show abstract

Olfactory childhood memories are particularly important for forming one's identity. However, we don't know how they exert their privileged influence and shape brain structure. To address this, we modeled childhood olfactory memory in mice based on a human survey indicating that our earliest olfactory memory arises from repeated positive experiences paired with a pleasant odorant. Accordingly, mice were exposed during childhood to a pleasant odorant in a playful environment. In adulthood, memory recall relied on neonatal-born granule cells in the olfactory bulb, as their optogenetic silencing impaired retrieval, and on increased functional connectivity in the reward system. With age, memory persistence depended on re-exposure to the childhood odorant and was associated with the disengagement of neonatal-born granule cells, alongside with strengthened limbic functional connectivity. Together, these findings identify neonatal neurons as a key substrate for encoding childhood olfactory memory and reveal dynamic reorganization of brain networks supporting its long-term significance.

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