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Chromosome-level genome sequence of the C4 grass Themeda triandra reveals karyotype orthology with sorghum and genetic variation in accessions adapted to diverse environments

Butler, J. B.; Humphreys, J. L.; Allnutt, T.; Jacob, V. K.; Chen, L.; Correa-Lozano, A.; Lopez-Jurado, J.; Foo, E.; Wright, I. J.; Smith, S. M.; Atwell, B. J.

2026-03-20 genomics
10.64898/2026.03.18.712786 bioRxiv
Show abstract

Themeda triandra (family Andropogoneae) is a pan-continental grass occurring throughout Australia. Recognising its remarkable adaptive capacity, we assembled the nuclear genome sequence of a diploid accession from eastern Australia, finding all ten chromosomes of the 755 Mb assembly highly syntenic with those of Sorghum bicolor. Genotyping and cytometry of range-wide accessions suggested several historical radiation events, with polyploids dominating in arid regions, apart from a notable diploid lineage from the arid north-west of Australia (PAN). A detailed comparison of PAN with two diploids from temperate south-eastern Australia through whole-genome resequencing revealed extensive copy number variation and polymorphism, with changes in genes for heat-shock proteins and flowering regulation reflecting their environmental origin. Exploration of the rich genetic diversity in T. triandra with respect to environmental adaptation is expected to benefit grassland management programs and enable introgression of novel genes into Andropogoneae crops for climate resilience.

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