Elucidating genes sufficient for viral entry into cells through sequential genome-wide CRISPR activation screens
Chai, T.; Wong, A.; Yin, Q.; von Creytz, I.; Weissman, J. S.; Saunders, R. A.; Prescott, J. B.; Loh, K. M.
Show abstract
A preeminent goal of virology is to discover cellular genes that mediate virus entry. Genome-wide loss-of-function screens can illuminate single genes necessary for virus entry, but are stymied by genetic redundancy. Here we report a genome-wide CRISPR activation screening strategy to discover single genes that are sufficient for viral entry into normally-uninfectable cells. Sequential rounds of viral infection vastly enhanced screening sensitivity. This sequential screening strategy was generalizable to two unrelated viruses--Ebola and rabies viruses--and could broadly accelerate the discovery of viral entry factors.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- CRISPR with Transcriptional Readout reveals influenza transcriptionis modulated by NELF and can precipitate an interferon response 97%
- Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy 96%
- Cross-reactive sarbecovirus antibodies induced by mosaic RBD-nanoparticles 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.