Comparative human embryo-mapping reveals neural bias of neuromesodermal progenitors in stem cell axial elongation models
Wang, Y.; Buzatu, R.; Herbermann, C.; Drukker, M.; Schroter, C.
Show abstract
Elongation of the human body axis is driven by the coordinated differentiation of neural and mesodermal tissues. Pluripotent stem cell-based organoid models recapitulate key aspects of axial elongation, but how they relate to the regional organization of axial development in the embryo remains unclear. Here, we map scRNA-seq from twelve stem cell-based axial organoids to a stage-matched embryo. Integrated transcriptomic and trajectory analyses show that human axial development involves three origins: forebrain neural progenitors, primitive streak mesoderm, and neuromesodermal progenitors (NMPs) with posterior potency. Axial organoids capture subsets of these origins, and contain NMPs with strong neural bias. A multivariate regression model linking pathway modulation to cell type frequencies revealed a key role of TGF-{beta} inhibition in the transition from anterior to posterior progenitors. Together, these findings clarify how the human body axis is partitioned into distinct domains by coordinated progenitor states and signaling.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Eomes restricts Brachyury functions at the onset of mammalian gastrulation 96%
- Single cell transcriptomics reveals chondrocyte differentiation dynamics in vivo and in vitro 95%
- Differential tissue deformability underlies shape divergence of the embryonic brain and spinal cord under fluid pressure 95%
Similar papers in this journal
- Axial Elongation of Caudalized Human Organoids Mimics Neural Tube Development 97%
- Tbx1 stabilizes differentiation of the cardiopharyngeal mesoderm and drives morphogenesis in the pharyngeal apparatus 95%
- TBXT dose sensitivity and the decoupling of nascent mesoderm specification from EMT progression in 2D human gastruloids 95%
Similar papers in this journal
- BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation 95%
- Cell-autonomous differentiation of human primed embryonic stem cells into trophoblastic syncytia through the nascent amnion-like cell state 95%
- Origin of Ewing sarcoma by embryonic reprogramming of neural crest to mesoderm 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.