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Molecular Transducers of Physical Activity Consortium (MoTrPAC): Initial Insights into the Dynamic Human Responses to Exercise

MoTrPAC Study Group, ; Brandt, A. R.; Fleg, J.; Goodpaster, B. H.; Jaeger, B.; Jin, C. A.; Johannsen, N. M.; Katz, D.; Keshishian, H.; Kohrt, W. M.; Kraus, W. E.; Lester, B.; Melanson, E. L.; Miller, M. E.; Montalvo, S.; Rejeski, W. J.; Shimly, S. M.; Smith, G. R.; Stowe, C. L.; Trappe, S.; AbouAssi, H.; Adams, N.; Amar, D.; Ashley, E.; Aslamy, A.; Bamman, M. M.; Belangee, A.; Bennett, W.; Bergman, B. C.; Bessesen, D. H.; Bodine, S. C.; Boyd, G.; Buford, T. W.; Burant, C. F.; Carnero, E. A.; Carr, S.; Chambers, T. L.; Chavez, C.; Chen, H.; Chen, S.-H.; Christle, J. W.; Claiborne, A.; Clark, N

2026-03-05 systems biology
10.64898/2026.03.02.705347 bioRxiv
Show abstract

The goal of the Molecular Transducers of Physical Activity Consortium (MoTrPAC) is to examine the physiological and molecular basis for health benefits in response to acute and chronic exercise. Prior to COVID-19 suspension, healthy, sedentary participants (N=206, 18-74y) were randomized to endurance exercise (N=80), resistance exercise (N=81), or non-exercise control (N=45) interventions. The prescribed vigorous acute endurance and resistance exercise bouts induced physiological and metabolic perturbations relative to resting homeostasis. The supervised chronic (3d/wk, 12wk) endurance or resistance training programs robustly improved several physiological parameters (i.e., VO2peak, muscular strength). Temporal biospecimen (blood, muscle, and adipose) collections and processing coupled to the acute exercise bouts were highly successful. In most cases, over 90% success was achieved for blood, muscle, and adipose samples. Endurance and resistance exercise induced distinct acute and chronic physiological responses, which provide a framework to interrogate the molecular basis for health adaptations to these two popular exercise modalities. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=200 SRC="FIGDIR/small/705347v1_ufig1.gif" ALT="Figure 1"> View larger version (65K): org.highwire.dtl.DTLVardef@c51f79org.highwire.dtl.DTLVardef@1954154org.highwire.dtl.DTLVardef@1f3f8c3org.highwire.dtl.DTLVardef@50ec08_HPS_FORMAT_FIGEXP M_FIG C_FIG

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