Super-resolution single-cell spatial atlas of plant de novo regeneration
Song, X.; Zhang, S.; Yue, Z.; Liu, Y.; Chen, S.; Niu, Y.; Shi, Y.; Yang, H.; Xu, L.; Liu, N.; Miao, Y.; Lv, M.; Li, J.; Wang, T.; Xu, M.; Sun, B.; Qiu, C.; Xu, R.; Wang, J.; Zhang, H.; Hou, S.; Li, G.; Chen, H.; Deng, X. W.; Li, B.
Show abstract
Plant de novo regeneration, a capacity absent in animals, relies on stem-cell-niche formation rather than pre-existing reservoirs. Growth hormones such as auxin and cytokinin, together with numerous genetic regulators, participate in regeneration, but the architectural principles coordinating extensive cellular reprogramming at the individual-cell scale remain elusive. Using super-resolution multimodal spatial transcriptomics to 1.16 million cells, we tracked tomato regeneration across temporal intervals from wounding to organogenesis. Datasets are available at http://www.single-cell-spatial.com.
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