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Early sex-specific organ transcriptional divergence without physiological differences in a murine model of fecal-induced peritonitis

Troitskaya, A.; Gill, S. E.; Manji, A.; Veldhuizen, R. A. W.; Batnyam, O.; Patterson, E. K.; Jahandideh, F.; Lalu, M. M.; Dwivedi, D. J.; Fox-Robichaud, A. E.; Liaw, P. C.; Cepinskas, G.; Mendelson, A. A.; McDonald, B.; Bourque, S. L.; Macala, K. F.; National Preclinical Sepsis Platform, The Canadian Critical Care Translational Biology Group, and Se,

2026-02-19 pathology
10.64898/2026.02.18.706550 bioRxiv
Show abstract

Sepsis is defined as a dysregulated response to infection, leading to life-threatening organ dysfunction that particularly affects parenchymal organs. Clinical studies remain inconclusive regarding the impact of biological sex on sepsis, and preclinical studies are predominantly performed in male animals. We examined early (8 h) septic responses in male and female mice using a fecal-induced peritonitis (FIP) model. Blood biochemical parameters, body temperature, and murine sepsis scores provided evidence of a septic response in animals randomized to FIP compared to controls, but showed no physiological differences between male and female mice. Transcriptomic analysis of the liver, kidney, and lung showed consistent inflammatory activation in response to sepsis as compared to controls. Notably, in the kidney and lung, female mice exhibited stronger immune activation and a heightened inflammatory response compared to males. Thus, biological sex differences in the septic response can be detected in early acute sepsis without apparent physiological differences.

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