Saline-free preparation for chronic in vivo imaging in adult Drosophila
Zhu, R.; Khorbtli, S.; Zhang, J.; Fu, Z.; Huang, C.
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Longitudinal brain imaging is essential for understanding neural mechanisms. Here, we present a saline-free, chronic preparation for repeated neural recording in adult Drosophila over multiple days. We describe steps for mounting flies, performing manual surgery on the head cuticle without external saline, and resealing the opening to create a transparent optical window. We demonstrate the utility of this approach by tracking single-neuron spiking and neuronal calcium dynamics over 7-10 days. This protocol is potentially applicable to other insect species. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=173 SRC="FIGDIR/small/706199v1_ufig1.gif" ALT="Figure 1"> View larger version (51K): org.highwire.dtl.DTLVardef@abeb34org.highwire.dtl.DTLVardef@deaf93org.highwire.dtl.DTLVardef@1d8fc24org.highwire.dtl.DTLVardef@91a696_HPS_FORMAT_FIGEXP M_FIG C_FIG
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