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Targeting FGFR signaling overcomes therapeutic resistance and immune evasion in oncogenic PIK3CA-driven serous-like endometrial cancer

Cheng, X.; Qian, C.; Holdridge, E.; Ananda, G.; Jiang, T.; Zhang, Y.; Ni, J.; Xie, S.; Gu, H.; Ji, R.; Ivanova, E. V.; Nucci, M. R.; Wang, Z.; Chen, K.; Kochupurakkal, B.; Freeman, G. J.; Shapiro, G. I.; Liu, J.; Konstantinopoulos, P. A.; Matulonis, U.; Zhao, J. J.

2026-02-17 cancer biology
10.64898/2026.02.16.706009 bioRxiv
Show abstract

Serous endometrial cancer (SEC) is an aggressive subtype of endometrial cancer (EC) with poor prognosis and limited treatment options. Here, we developed a clinically relevant, immunocompetent serous-like mouse model incorporating oncogenic PIK3CA mutation, Trp53 loss, and MYC overexpression. Using this model together with human EC cell lines, patient-derived organoids (PDOs), xenografts, and patient datasets, we investigated mechanisms underlying resistance to PI3K-targeted therapy. Single-cell profiling reveals that FGFR1/2 upregulation associates with intrinsic resistance, whereas FGFR3 characterizes acquired resistance. Dual FGFR and PI3K inhibition produced superior tumor control compared with either agent alone. Mechanistically, FGFR signaling promotes immune evasion by downregulating MHC-I/HLA-mediated antigen presentation and enriching M2-type tumor-associated macrophages. FGFR inhibition reversed these changes and synergized with anti-PD-1 therapy to enhance antitumor immune responses and establish durable immune memory. Collectively, these findings identify FGFR signaling as a key driver of therapeutic resistance and immune escape in SEC and support FGFR-targeted combination strategies.

Published in Nature Communications (predicted rank #1) · training set

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