Exploring the effects of Golgi Reassembly and Stacking Proteins in lipid membranes
Kava, E.; Malacrida, L. S.; Diaz, M.; Itri, R.; Costa-Filho, A. J.
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Golgi Reassembly and Stacking Proteins (GRASP) have been associated with Golgi-ribbon structure and unconventional vesicular protein secretion. In performing these functions, GRASPs consistently interact with membranes. The presence of lipid modifications, such as myristoylation, is a crucial consideration for obtaining detailed information about the interactions between GRASPs and membranes. Nonetheless, it has been overlooked in the literature so far. Here, we describe a reconstitution protocol for myristoylated human GRASP65 and GRASP55 in lipid model membranes, enabling investigation of their interactions using techniques ranging from structural characterization to spectroscopy and microscopy. Our results showed that myristoyl-anchored GRASPs can influence membrane dynamics, suggesting a possible role for their disordered SPR domain in this interaction.
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