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Sex-specific control of feeding and defensive behaviors by MC3R neurons in the bed nuclei of the stria terminalis

Bedenbaugh, M. N.; Doyle, M. A.; Edwards, C. M.; Petersen, N.; Srisai, D.; Hawkins, S. H.; Thomas-Low, N. D.; Mendoza-Romero, H.; Brown, J. A.; Vardy, A. S.; Winder, D. G.; Simerly, R. B.

2026-02-09 neuroscience
10.64898/2026.02.08.702109 bioRxiv
Show abstract

The bed nuclei of the stria terminalis (BST) is a nuclear complex that coordinates neuroendocrine, autonomic, and behavioral responses associated with maintaining homeostasis. Here, we demonstrate that melanocortin 3 receptor (MC3R) neurons in the BST (BSTMC3R) play a key role in modulating feeding behaviors and responses to stressful events. BSTMC3R neurons are primarily GABAergic and colocalize with neuropeptides known to regulate feeding and affective behaviors. Chemogenetic activation of BSTMC3R neurons causes reduced feeding, particularly in males. BSTMC3R neurons are also robustly activated by stress and can control responses to stress and defensive behaviors in both sexes. Whole-brain monosynaptic rabies tracing identified multiple sexually dimorphic inputs to BSTMC3R neurons that may contribute to observed functional sexual dimorphisms. Altogether, these data reveal that BSTMC3R neurons participate in sexually dimorphic circuits that influence feeding and defensive behaviors, responses to stress, and may represent a potential therapeutic target for stress- and eating-related disorders.

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