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Adaptive Optics Fluorescence Lifetime Imaging Ophthalmoscopy for Single-Cell-Resolved In Vivo Metabolic and Structural Imaging of the Human Retinal Pigment Epithelium
2026-02-09
ophthalmology
Title + abstract only
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Fluorescence lifetime imaging ophthalmoscopy permits in vivo assessment of retinal metabolism but has remained limited by insufficient cellular resolution in the human eye. Here we present adaptive optics-enhanced fluorescence lifetime imaging ophthalmoscopy (AOFLIO), a method for single-cell-resolved, in vivo structural and metabolic imaging of the human retinal pigment epithelium (RPE). Through real-time correction of ocular wavefront aberrations, precisely synchronized adaptive optics reflect...
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