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Orofacial behaviors, not eye movements, govern neural activity in mouse visual cortex

Syeda, A.; Nunez-Ochoa, M. A.; Zhong, L.; Pachitariu, M.; Stringer, C.

2026-02-05 neuroscience
10.64898/2026.02.04.703800 bioRxiv
Show abstract

Neural activity in mouse primary visual cortex (V1) correlates strongly with orofacial movements. Such strong modulation has not been found in the primate visual cortex during eye fixation [1], which led to the suggestion that the modulation may primarily depend on eye movements in both species [2]. Here we examined the influence of eye movements on neural activity in mouse visual cortex both in complete darkness and in the presence of different types of visual input. In all cases, we found that eye movements explain a smaller fraction of neural activity variance compared to orofacial behaviors. Additionally, we found that eye movements were correlated to orofacial movements, such as whisking and sniffing, and thus may be indirectly correlated to neural activity. These results further emphasize the impact of movement signals on mouse visual cortex during free viewing behavior.

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