S-nitrosylation of the CLIP-protease, Persephone, Regulates Drosophila innate immunity
Homem, R. A.; Kanchanawatee, K.; Li, Y.; Chang, T.-H.; Zheng, J.; Finnegan, D. J.; Loake, G. J.
Show abstract
Nitric oxide (NO) modulates innate immunity, but its molecular targets in Drosophila melanogaster are largely undefined. A key mechanism of NO signalling is S-nitrosylation, the modification of cysteine thiols. The homeostasis of S-nitrosylation is maintained by S-nitrosoglutathione reductase (Gsnor), encoded by the fdh gene in D. melanogaster. As reduced Gsnor activity enhances pathogen sensitivity in plants, we investigated its role in D. melanogaster. Here, we show that flies lacking fdh exhibit increased susceptibility to the fungus Beauveria bassiana and the bacterium Staphylococcus aureus, pathogens combatted by the Toll pathway. This immune deficiency correlates with impaired Toll-dependent antimicrobial peptide expression. We demonstrate that the Toll pathway protease Persephone (Psh) is S-nitrosylated in vivo and that loss of Gsnor prevents its proteolytic activation following infection. We propose a model where Gsnor-mediated regulation of NO is essential for Toll activation, preventing excessive S-nitrosylation of Psh and ensuring a robust immune response.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Innate behavior sequence progression by peptide-mediated interorgan crosstalk 96%
- The deubiquitylating enzyme Fat Facets promotes Fat signalling and restricts tissue growth 96%
- The Drosophila proventriculus lacks stem cells but compensates for age-related cell loss via endoreplication-mediated cell growth 95%
Similar papers in this journal
- Molecular characterization of gustatory second-order neurons reveals integrative mechanisms of gustatory and metabolic information 95%
- Muscle function and homeostasis require macrophage-derived cytokine inhibition of AKT activity in Drosophila 95%
- Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila 95%
Similar papers in this journal
Similar papers in this journal
- Paired C-type lectin receptors mediate specific recognition of divergent oomycete pathogens in C. elegans. 95%
- Infection-Induced Elevation of Gut Glycosaminoglycans Fosters Microbiota Expansion in Drosophila melanogaster 94%
- Insulin/insulin-like growth factor signaling pathway promotes higher fat storage in Drosophila females 94%
Similar papers in this journal
- Resistance to host antimicrobial peptides mediates resilience of gut commensals during infection and aging in Drosophila 95%
- Neuronal IL-17 controls C. elegans developmental diapause through CEP-1/p53 95%
- Structural modeling reveals the allosteric switch controlling the chitin utilization program of Vibrio cholerae 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.