Opposing regulation by Rev1 of DNA polymerase zeta activity on damaged versus undamaged DNA

The Rev1 deoxycytidyl transferase functions as a scaffold protein for DNA polymerase {zeta} (Pol {zeta})-mediated translesion synthesis (TLS). Biochemical studies with yeast enzymes indicate that Rev1 plays a dual regulatory role in TLS, stimulating Pol {zeta} activity at sites of damage but inhibiting its activity on undamaged DNA. An evolutionary conserved N-terminal alpha-helical motif (M1), located 10-20 amino acids upstream of Rev1s single BRCT domain, is required for the inhibitory activity of Rev1 on undamaged DNA. Mutations in the M1 motif result in a stimulation of Pol {zeta} replication activity on both undamaged and damaged DNA. Yeast cells carrying a REV1 mutant lacking the M1 motif, show a significant increase in mutation track length, without significantly affecting overall spontaneous mutation rates. The regulatory activity of Rev1 is independent of its catalytic activity. However, it requires that Rev1-Pol {zeta} is a stable complex, and that this complex is coordinated by the replication clamp PCNA. GRAPHICAL ABSTRACT O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=83 SRC="FIGDIR/small/700666v1_ufig1.gif" ALT="Figure 1"> View larger version (10K): org.highwire.dtl.DTLVardef@9b40dorg.highwire.dtl.DTLVardef@10bf5d1org.highwire.dtl.DTLVardef@376ff4org.highwire.dtl.DTLVardef@1970db8_HPS_FORMAT_FIGEXP M_FIG C_FIG