Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants
Mendez Diaz, F.; Huo, J.; Corbett, K. D.
Show abstract
In prophase of meiosis I, the proteinaceous chromosome axis provides a scaffold for the compaction of chromosomes into a linear loop array, controls the formation of interhomolog crossovers, and finally becomes integrated into the synaptonemal complex after crossovers have formed. Despite its fundamental importance, how the proteins of the meiotic chromosome axis - meiotic HORMADs, axis core proteins, and cohesin complexes - self-assemble with one another is incompletely understood. In particular, it remains unknown how cohesin complexes interact with other axis components. Here, we combine genetics in S. cerevisiae, AlphaFold-based protein interaction screens, and biochemical assays to reveal that the S. cerevisiae axis core protein Red1 binds the C-terminal winged helix domain of cohesins meiosis-specific subunit Rec8. We find that this interaction is conserved across fungi and plants, but not in mammals, suggesting that different eukaryotic phyla use distinct protein-protein interfaces to assemble the chromosome axis.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Moss BRCA2 lacking canonical DNA binding domain promotes efficient homologous recombination and binds to DNA 96%
- Mer3 helicase protects early crossover intermediates from STR complex disassembly during meiosis 95%
- Rec8 cohesin-mediated axis-loop chromatin architecture is required for meiotic recombination 95%
Similar papers in this journal
- Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase 96%
- Relocation of rDNA repeats for repair is dependent on SUMO-mediated nucleolar release by the Cdc48/p97 segregase 96%
- Divergence in a Eukaryotic Transcription Factor's co-TF Dependence Involves Multiple Intrinsically Disordered Regions 95%
Similar papers in this journal
Similar papers in this journal
- Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination 96%
- Multivalent interaction of ESCO2 with the replication machinery is required for cohesion 95%
- CtIP promotes the motor activity of DNA2 to accelerate long-range DNA end resection 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.