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Quality control of blood plasma for mass-spectrometry proteomic research

Vakaryuk, A. V.; Butenko, I. O.; Kitsilovskaya, N. A.; Kovalenko, A. V.; Gremyacheva, V. D.; Kozhemyakin, G. L.; Lebedeva, A. A.; Baraboshkin, N. M.; Fedorov, O. V.; Govorun, V. M.

2026-01-16 systems biology
10.64898/2026.01.15.699696 bioRxiv
Show abstract

Mishandling of samples during blood plasma preparation may lead to biases in proteomic analysis and false interpretation of data. Previous plasma proteome profiling study (Geyer, Mann et al., 2019) described proteins that may serve to indicate poor plasma sample quality. We have established a targeted quantitative method for three prominent panels of markers proposed by previous study for exclusion of hemolyzed and coagulation samples. We conducted a model experiment similar to Geyer, Mann et al., with both panoramic and targeted detection and investigated the feasibility of application internal control for hemolysis in targeted proteomic analysis by using incorrectly collected plasma. We propose to include plasma sample hemolysis assessment into standard MRM analysis and associated sample exclusion criteria.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.