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Analytical Sensitivity of the BOSCH Vivalytic Molecular Assay for Detection of Monkeypox Virus Clade Ib

Escadafal, C.; Adea, K.; Mbala, P.; Eckerle, I.

2026-01-11 infectious diseases
10.64898/2026.01.07.25342807 medRxiv
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We evaluated the BOSCH Vivalytic MPXV assay using serial dilutions of monkeypox virus clade Ib, a new offshoot of clade I identified in 2023. The assay detected viral DNA down to [~]100 copies/mL demonstrating comparable analytical sensitivity to our in-house reference PCR and to other commercial platform-based mpox molecular assays.

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