An anti-aggregation region of the SGS3 N-terminal IDR is essential for secondary siRNA biogenesis
Iwakawa, H.-o.; Fujimoto, Y.; Sakurai, Y.; Kowada, R.; Shoji, K.; Yoshikawa, M.
Show abstract
Secondary siRNA biogenesis amplifies small RNA signals from target transcripts and plays a pivotal role in plant development and defense responses. The RNA-binding protein SGS3 is essential for this pathway, recruiting RNA-dependent RNA polymerase 6 (RDR6) to Argonaute-small RNA-bound targets. The N-terminal intrinsically disordered region (IDR) of SGS3, which contains a prion-like domain (PrLD), has been reported to drive liquid-liquid phase separation, forming siRNA bodies, and to be required for secondary siRNA production. However, the molecular mechanism by which the N-terminal IDR contributes to secondary siRNA production remains unclear. Here, using in vitro reconstitution and in planta assays, we show that the N-terminal IDR comprises two functional modules: the PrLD and a negatively charged region (NCR). The PrLD is required for phase separation and siRNA body formation but is dispensable for secondary siRNA production. In contrast, mutations in the NCR caused SGS3 to form abnormally large cytoplasmic assemblies and markedly impaired secondary siRNA production. These results suggest that the N-terminal IDR helps maintain SGS3 in a functional, soluble state that supports efficient secondary siRNA biogenesis.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- SERRATE interacts with the nuclear exosome targeting (NEXT) complex to degrade primary miRNA precursors in Arabidopsis 95%
- Accessory proteins increase the efficiency of RNA editing by Arabidopsis chloroplast editosomes 94%
- Functional specialization of monocot DCL3 and DCL5 proteins through the evolution of the PAZ domain 94%
Similar papers in this journal
- A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination 95%
- Edge strand of Escherichia coli BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways 94%
- The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant specific motif and acts in a partially redundant manner with the tonoplast enzyme 94%
Similar papers in this journal
- Characterization of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex 95%
- High-pressure sprayed siRNAs trigger influence the efficiency but not the profile of transitive silencing 94%
- Functional requirement of the Arabidopsis importin-α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.