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Structural and functional evidence for ephaptic control of Purkinje cell spike timing by networks of molecular layer interneurons

Norton, A.; Lackey, E. P.; Ozturk, S.; Gaynor, C. S.; Ediger, S.; Lee, W.-C. A.; Hull, C. A.; Regehr, W. G.

2025-12-29 neuroscience
10.64898/2025.12.28.696768 bioRxiv
Show abstract

Axon collaterals of type 1 molecular layer interneurons (MLI1s) contribute to pinceaux that engulf the initial segments of Purkinje cell (PC) axons and generate extracellular signals that ephaptically inhibit PCs. Here we show that a remarkably large number of MLI1s ([~]50) contribute to each pinceau, and that this allows networks of synchronously firing MLI1s to use ephaptic signals to control the precise timing of PC firing in vivo.

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