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Single-Cell and TCR Profiling across Tissues Reveals GZMK⁺CD8⁺ T Cells as Drivers of Fibrosis in IPF

2025-12-22 respiratory medicine Title + abstract only
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Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease with limited therapies and poorly defined cross-tissue immune mechanisms. We performed single-cell RNA sequencing and TCR profiling of paired lung, lymph node, and peripheral blood samples from patients with IPF, combined with functional coculture assays and mouse model. We identified GZMK-high CD8 T cells enriched in fibrotic lungs, displaying inflammatory but low-cytotoxic features. TCR and trajectory analyses indicated t...

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