Engineering signal peptide-mediated recombinant protein secretion in Komagataeibacter rhaeticus for biological Engineered Living Material applications
Vannas, J.; Singh, A.; Hannikainen, B.; de Assis, M. A.; Ledesma-Amaro, R.; Ellis, T.; Mangayil, R.
Show abstract
Bacterial nanocellulose (BC) from Komagataeibacter spp. is an ideal scaffold for biological Engineered Living Materials (bioELMs). Engineering Komagataeibacter to simultaneously synthesize and functionalize BC could transform current workflows, but protein secretion in this organism remains poorly understood. Here, we demonstrate recombinant protein secretion in K. rhaeticus iGEM by leveraging its genome-encoded Sec-translocase. Using secretome analysis to identify native Sec signal peptides (SPs), secretion efficiencies of mScarlet and {beta}-lactamase variant libraries were benchmarked under pellicle-forming and non-pellicle conditions. Inducible expression eliminated metabolic burden and enabled secretion without cell lysis, as confirmed by fluorescence and scanning electron microscopy. Comparative analyses revealed strong condition-dependent variations in secretion performance, with native SPs exhibiting higher efficiency under pellicle-forming conditions. Finally, BC functionalization using secreted {beta}-lactamase was demonstrated, with no detrimental effects on the BC material. This work establishes the first comparative framework for SP-mediated secretion in Komagataeibacter and provides a foundation for next-generation BC-based bioELMs.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- LyGo: A platform for rapid screening of lytic polysaccharide monooxygenase production 96%
- Komagataeibacter tool kit (KTK): a modular cloning system for multigene constructs and programmed protein secretion from cellulose producing bacteria 95%
- Signal peptide efficiency: from high-throughput data to prediction and explanation 95%
Similar papers in this journal
- Thermophilic carboxylesterases from hydrothermal vents of the volcanic island of Ischia active on synthetic and biobased polymers and mycotoxins 94%
- A Multi-Tetracycline Responsive Induction System for Gene Expression in Bacillus subtilis 94%
- Insights in the complex DegU, DegS, Spo0A regulation system of Paenibacillus polymyxa by CRISPR-Cas9-based targeted point mutations 94%
Similar papers in this journal
- Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering 94%
- Genetic Optimisation of Bacteria-Induced Calcite Precipitation in Bacillus subtilis 93%
- Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus 93%
Similar papers in this journal
- Surpassing thermodynamic, kinetic, and stability barriers to isomerization catalysis for tagatose biosynthesis 97%
- Temporal gene regulation enables controlled expression of gas vesicles and preserves bacterial viability 95%
- Genome-scale metabolic rewiring to achieve predictable titers rates and yield of a non-native product at scale 94%
Similar papers in this journal
- Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness 93%
- An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics 92%
- Making plants into cost-effective bioreactors for highly active antimicrobial peptides 89%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.