Genetic associations of eosinophilic granulomatosis with polyangiitis in the Japanese population: Exploring similarities and differences with European populations

ObjectivesEosinophilic granulomatosis with polyangiitis (EGPA) is classified as a subtype of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). A recent genome-wide association study (GWAS) of EGPA in populations of European ancestry reported several susceptibility loci. In view of population differences in genetic factors of AAV, we made an attempt to examine the contribution of these loci to EGPA in a Japanese population. MethodsThe EGPA associated variants in LPP, TSLP, IRF1/IL5, HLA, CDK6, 10p14 and 12q21 in European populations were analyzed in a total of 103 Japanese patients with EGPA, including 48 ANCA-positive and 45 ANCA-negative patients. Allele frequency data on approximately 60,000 Japanese individuals from the Japanese Multi Omics Reference Panel (jMorp) served as the controls. ResultsAn increasing tendency in TSLP rs1837253 C allele was observed in total EGPA, myeloperoxidase (MPO)-ANCA-positive EGPA and ANCA-negative EGPA. In addition, association of HLA-DRB1*07:01 was observed in total EGPA and MPO-ANCA-positive EGPA. These findings supported the previous findings in the European populations. On the other hand, unlike in the European populations, association of DRB1*09:01 was observed in Japanese EGPA. With respect to amino acid residues in HLA-DR{beta}1, Val 78 encoded by DRB1*07:01 and *09:01 was associated with total EGPA and MPO-ANCA-positive EGPA with genome-wide significance (P<5.0E-08). Association with the other loci was not observed in Japanese EGPA. ConclusionTSLP was suggested to be associated with EGPA both in the European and Japanese populations regardless of the ANCA status. In HLA-DRB1, both shared and population-specific susceptibility alleles were observed. What is already known on this topicO_LISusceptibility genes to eosinophilic granulomatosis with polyangiitis (EGPA) have been reported by a genome-wide association study (GWAS) in populations of European ancestry. C_LIO_LIIn non-European populations, GWAS of EGPA has not been reported and genetic factors contributing to the development of EGPA are largely unknown. C_LI What this study addsO_LITSLP rs1837253C and HLA-DRB1*07:01 were shown to be associated with EGPA both in European and Japanese populations. C_LIO_LIHLA-DRB1*09:01 was shown to be associated with EGPA in the Japanese, but not in the European populations. C_LI How this study might affect research, practice or policyO_LIThis study showed that TSLP is a shared susceptibility gene to EGPA in the European and Japanese populations, implicating its significance as a molecular target as well as a potential biomarker for response to IL-5 targeted therapy across populations. C_LIO_LIPopulation differences are observed in allelic associations other than TSLP, which should be taken into consideration in the process of drug and biomarker development for EGPA. C_LI